Electrochemical aptasensor based on Ce3NbO7/CeO2@Au hollow nanospheres by using Nb.BbvCI-triggered and bipedal DNA walker amplification strategy for zearalenone detection.
J Hazard Mater
; 438: 129491, 2022 09 15.
Article
in En
| MEDLINE
| ID: mdl-35785741
ABSTRACT
Herein, an electrochemical aptasensor combining Nb.BbvCI-triggered bipedal DNA walking strategy was constructed for ultrasensitive assay of zearalenone (ZEN). The aptasensor used Ce3NbO7/CeO2 @Au hollow nanospheres as electrode modification material and PdNi@MnO2/MB as the signal label. Importantly, the Ce3NbO7/CeO2 synthesized by hydrothermal method were combined with Au nanoparticles and applied to the electrode surface. The as-prepared Ce3NbO7/CeO2 @Au possessed a large surface area, excellent electrical conductivity, stability and more binding sites. PdNi@MnO2 with high specific surface area and porosity combined with molecule methylene blue (MB) was introduced into electrodes as the signal label. The proposed aptasensor utilized the advantages of specific recognition of aptamers and target molecules to release bipedal DNA walker (w-DNA), and then the w-DNA was triggered by Nb.BbvCI and entered the cycle to release more signal probes. The feasibility of this strategy was recorded by the differential pulse voltammetry (DPV) method. Under the optimized conditions, the electrochemical aptasensor exhibited a wide linear dynamic range from 1 × 10-4 to 1 × 103 ng mL-1 with a low detection limit of 4.57 × 10-6 ng mL-1. Moreover, the aptasensor had high selectivity, good stability, excellent repeatability and provided an effective method for the trace detection of ZEN in real samples.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Zearalenone
/
Biosensing Techniques
/
Aptamers, Nucleotide
/
Metal Nanoparticles
/
Nanospheres
Type of study:
Diagnostic_studies
Language:
En
Journal:
J Hazard Mater
Journal subject:
SAUDE AMBIENTAL
Year:
2022
Type:
Article