Your browser doesn't support javascript.
loading
Presence of quorum sensing system, virulence genes, biofilm formation and relationship among them and class 1 integron in carbapenem-resistant clinical Pseudomonas aeruginosa isolates.
Baskan, Ceren; Siriken, Belgin; Tüfekci, Enis Fuat; Kilinç, Çetin; Ertürk, Ömer; Erol, Irfan.
Affiliation
  • Baskan C; Sabuncuoglu Serefeddin Health Services, Vocational School, Serefeddin Sabuncuoglu Health School, Amasya University, Amasya, Turkey. ceren.yavuz@amasya.edu.tr.
  • Siriken B; Department of Aquatic Animal Diseases, Faculty of Veterinary Medicine, Ondokuz Mayis University, Samsun, Turkey.
  • Tüfekci EF; Department of Medical Microbiology, Faculty of Medicine, Kastamonu University, Kastamonu, Turkey.
  • Kilinç Ç; Department of Medical Microbiology, Faculty of Medicine, Kastamonu University, Kastamonu, Turkey.
  • Ertürk Ö; Department of Molecular Biology and Genetics, Faculty of Science and Arts, Ordu University, Ordu, Turkey.
  • Erol I; Faculty of Health Sciences, Eastern Mediterranean University, Famagusta TRNC Via Mersin, Mersin, Turkey.
Arch Microbiol ; 204(8): 464, 2022 Jul 08.
Article in En | MEDLINE | ID: mdl-35802194
ABSTRACT
Carbapenems are the most effective agents for treating clinical P. aeruginosa (PsA) infections. During an infection, a quorum-sensing (QS) system and its regulating virulence genes have a great role. The aim of the study was to detect the presence of a las and rhl QS system and related virulence genes, biofilm formation and a class 1 (Cls1) integron. A total of 52 carbapenem-resistant PsA (CRPsA) isolates obtained from Kastamonu, Turkey was analyzed. For the isolation and identification of CRPsA isolates, a conventional culture method, an automated VITEK-2 compact system, and oprL gene-based molecular technique were applied. The two QS system genes were detected in 51 (98.1%), and co-existed of four two QS system genes (lasI/R and rhIl/R genes) were determined in 41 (78.8%) of the isolates. algD, lasB, toxA and aprA genes were detected in between 46.1 and 88.5%, and co-existence of four two QS system genes with four virulence genes were detected in 40.4% of the isolates. Biofilm formation using microtiter plate assay and slime production using Congo Red Agar and Cls1 integron were determined in 84.6%, 67.3% and 51.9% of the isolates, respectively. According to statistical analyses results, there was a significant positive correlation (p < .10) between the las and the rhl systems and a strongly and positive correlation (p < .01 or p < .05) between the rhl system-three virulence genes and slime production-and among some virulence genes. In conclusion, the CRPsA isolates tested in the study are highly virulent and QS systems have a significant role in pathogenesis.
Subject(s)
Key words

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Pseudomonas aeruginosa / Virulence Factors / Integrons / Quorum Sensing Type of study: Prognostic_studies Language: En Journal: Arch Microbiol Year: 2022 Type: Article Affiliation country: Turkey

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Pseudomonas aeruginosa / Virulence Factors / Integrons / Quorum Sensing Type of study: Prognostic_studies Language: En Journal: Arch Microbiol Year: 2022 Type: Article Affiliation country: Turkey