Uronic acid carbazole assay and cetylpyridinium chloride titration depend on the chondroitin sulfate molecular weight.
Anal Biochem
; 655: 114848, 2022 10 15.
Article
in En
| MEDLINE
| ID: mdl-35948059
ABSTRACT
Chondroitin sulfate (CS) of various molecular weight (MW), up to â¼3 kDa, were produced and tested for uronic acid carbazole assay and cetylpyridinium chloride (CPC) titration showing an evident decrease in the assays depending on the CS MW. The described results for uronic acid assay by carbazole reaction and CPC titration of CS poses the problem to know the MW values before their application and to use comparable standards to obtain reliable results. Otherwise, the related quantitative data can be affected by a great error and fake certificate of analysis.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Uronic Acids
/
Chondroitin Sulfates
Language:
En
Journal:
Anal Biochem
Year:
2022
Type:
Article
Affiliation country:
Italy