Citrus Protoplast Isolation and Plant Regeneration Through Somatic Embryogenesis.
Methods Mol Biol
; 2527: 111-126, 2022.
Article
in En
| MEDLINE
| ID: mdl-35951187
ABSTRACT
Protoplasts are an attractive explant source for biotechnological tools widely used on citrus genetic improvement, such as somatic hybridization and direct genetic transformation. These delicate and responsive materials are subjected to cell proliferation induction and differentiation of somatic embryos which further regenerate into entire plants. The isolation of viable protoplasts followed by regeneration of plants through somatic embryogenesis is an important methodology for breeding applications. The methods presented here can also be used as a reference for protoplast work in other species, followed by protocol optimization for different species/genotypes.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Protoplasts
/
Citrus
Language:
En
Journal:
Methods Mol Biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2022
Type:
Article
Affiliation country:
Brazil