Prevalence and genetic analysis of Enterobius vermicularis in schoolchildren in lower northern Thailand.
Parasitol Res
; 121(10): 2955-2965, 2022 Oct.
Article
in En
| MEDLINE
| ID: mdl-35972547
ABSTRACT
Enterobius vermicularis, a nematode parasite with a global distribution causes enterobiasis in schoolchildren and is considered a neglected parasite. An understanding of the prevalence and genetic diversity of enterobiasis is crucial for appropriate control measures. Therefore, the objectives of this research were to study the prevalence and genetic diversity of E. vermicularis in schoolchildren from lower northern Thailand, based on cytochrome c oxidase subunit I (COI) and internal transcribed spacer 2 (ITS2) sequences. Using the scotch tape technique, 7.4% (188/2544) of schoolchildren from 21 primary schools were found positive for E. vermicularis eggs, which is a relatively low infection rate. Phylogenetic trees of partial COI sequences (397 bp) revealed similar topologies using maximum likelihood (ML) and neighbor-joining (NJ) methods and identified E. vermicularis type A (105 sequences) and B (1 sequence). Haplotype network analysis of the COI sequences demonstrated a high haplotype diversity (Hd = 0.9028). In contrast, phylogenetic analysts of a 343 bp region of the ITS2 locus (52 sequences) revealed a monophyletic group. More sequence analyses of E. vermicularis from humans and other hosts in Thailand are necessary to better understand the genetic diversity of this parasite.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Enterobiasis
/
Enterobius
Type of study:
Prevalence_studies
/
Risk_factors_studies
Limits:
Animals
/
Child
/
Humans
Country/Region as subject:
Asia
Language:
En
Journal:
Parasitol Res
Journal subject:
PARASITOLOGIA
Year:
2022
Type:
Article
Affiliation country:
Thailand