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A human fetal lung cell atlas uncovers proximal-distal gradients of differentiation and key regulators of epithelial fates.
He, Peng; Lim, Kyungtae; Sun, Dawei; Pett, Jan Patrick; Jeng, Quitz; Polanski, Krzysztof; Dong, Ziqi; Bolt, Liam; Richardson, Laura; Mamanova, Lira; Dabrowska, Monika; Wilbrey-Clark, Anna; Madissoon, Elo; Tuong, Zewen Kelvin; Dann, Emma; Suo, Chenqu; Goh, Isaac; Yoshida, Masahiro; Nikolic, Marko Z; Janes, Sam M; He, Xiaoling; Barker, Roger A; Teichmann, Sarah A; Marioni, John C; Meyer, Kerstin B; Rawlins, Emma L.
Affiliation
  • He P; Wellcome Sanger Institute, Hinxton, Cambridge CB10 1SA, UK; European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI), Wellcome Genome Campus, Cambridge, UK.
  • Lim K; Wellcome Trust/CRUK Gurdon Institute, Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge CB2 1QN, UK.
  • Sun D; Wellcome Trust/CRUK Gurdon Institute, Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge CB2 1QN, UK.
  • Pett JP; Wellcome Sanger Institute, Hinxton, Cambridge CB10 1SA, UK.
  • Jeng Q; Wellcome Trust/CRUK Gurdon Institute, Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge CB2 1QN, UK.
  • Polanski K; Wellcome Sanger Institute, Hinxton, Cambridge CB10 1SA, UK.
  • Dong Z; Wellcome Trust/CRUK Gurdon Institute, Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge CB2 1QN, UK.
  • Bolt L; Wellcome Sanger Institute, Hinxton, Cambridge CB10 1SA, UK.
  • Richardson L; Wellcome Sanger Institute, Hinxton, Cambridge CB10 1SA, UK.
  • Mamanova L; Wellcome Sanger Institute, Hinxton, Cambridge CB10 1SA, UK.
  • Dabrowska M; Wellcome Sanger Institute, Hinxton, Cambridge CB10 1SA, UK.
  • Wilbrey-Clark A; Wellcome Sanger Institute, Hinxton, Cambridge CB10 1SA, UK.
  • Madissoon E; Wellcome Sanger Institute, Hinxton, Cambridge CB10 1SA, UK; European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI), Wellcome Genome Campus, Cambridge, UK.
  • Tuong ZK; Wellcome Sanger Institute, Hinxton, Cambridge CB10 1SA, UK; Molecular Immunity Unit, University of Cambridge Department of Medicine, Cambridge, UK.
  • Dann E; Wellcome Sanger Institute, Hinxton, Cambridge CB10 1SA, UK.
  • Suo C; Wellcome Sanger Institute, Hinxton, Cambridge CB10 1SA, UK; Department of Paediatrics, Cambridge University Hospitals, Hills Road, Cambridge CB2 0 QQ, UK.
  • Goh I; Biosciences Institute, Newcastle University, Newcastle upon Tyne, NE2 4HH, UK.
  • Yoshida M; Lungs for Living Research Centre, UCL Respiratory, University College London, London, UK.
  • Nikolic MZ; Lungs for Living Research Centre, UCL Respiratory, University College London, London, UK.
  • Janes SM; Lungs for Living Research Centre, UCL Respiratory, University College London, London, UK.
  • He X; John van Geest Centre for Brain Repair, Department of Clinical Neurosciences and Wellcome-MRC Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK.
  • Barker RA; John van Geest Centre for Brain Repair, Department of Clinical Neurosciences and Wellcome-MRC Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK.
  • Teichmann SA; Wellcome Sanger Institute, Hinxton, Cambridge CB10 1SA, UK; Department of Physics, Cavendish Laboratory, University of Cambridge, Cambridge CB3 0HE, UK.
  • Marioni JC; Wellcome Sanger Institute, Hinxton, Cambridge CB10 1SA, UK; European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI), Wellcome Genome Campus, Cambridge, UK; Cancer Research UK Cambridge Institute, University of Cambridge, Cambridge, UK.
  • Meyer KB; Wellcome Sanger Institute, Hinxton, Cambridge CB10 1SA, UK.
  • Rawlins EL; Wellcome Trust/CRUK Gurdon Institute, Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge CB2 1QN, UK. Electronic address: e.rawlins@gurdon.cam.ac.uk.
Cell ; 185(25): 4841-4860.e25, 2022 Dec 08.
Article in En | MEDLINE | ID: mdl-36493756
ABSTRACT
We present a multiomic cell atlas of human lung development that combines single-cell RNA and ATAC sequencing, high-throughput spatial transcriptomics, and single-cell imaging. Coupling single-cell methods with spatial analysis has allowed a comprehensive cellular survey of the epithelial, mesenchymal, endothelial, and erythrocyte/leukocyte compartments from 5-22 post-conception weeks. We identify previously uncharacterized cell states in all compartments. These include developmental-specific secretory progenitors and a subtype of neuroendocrine cell related to human small cell lung cancer. Our datasets are available through our web interface (https//lungcellatlas.org). To illustrate its general utility, we use our cell atlas to generate predictions about cell-cell signaling and transcription factor hierarchies which we rigorously test using organoid models.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Fetus / Lung Type of study: Prognostic_studies Limits: Humans Language: En Journal: Cell Year: 2022 Type: Article Affiliation country: United kingdom
Fulltext
Print
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Fetus / Lung Type of study: Prognostic_studies Limits: Humans Language: En Journal: Cell Year: 2022 Type: Article Affiliation country: United kingdom