Thermo-Responsive Nanocomposite Bioink with Growth-Factor Holding and its Application to Bone Regeneration.
Small
; 19(9): e2203464, 2023 03.
Article
in En
| MEDLINE
| ID: mdl-36526612
ABSTRACT
Three-dimensional (3D) bioprinting, which is being increasingly used in tissue engineering, requires bioinks with tunable mechanical properties, biological activities, and mechanical strength for in vivo implantation. Herein, a growth-factor-holding poly(organophosphazene)-based thermo-responsive nanocomposite (TNC) bioink system is developed. The mechanical properties of the TNC bioink are easily controlled within a moderate temperature range (5-37 °C). During printing, the mechanical properties of the TNC bioink, which determine the 3D printing resolution, can be tuned by varying the temperature (15-30 °C). After printing, TNC bioink scaffolds exhibit maximum stiffness at 37 °C. Additionally, because of its shear-thinning and self-healing properties, TNC bioinks can be extruded smoothly, demonstrating good printing outcomes. TNC bioink loaded with bone morphogenetic protein-2 (BMP-2) and transforming growth factor-beta1 (TGF-ß1), key growth factors for osteogenesis, is used to print a scaffold that can stimulate biological activity. A biological scaffold printed using TNC bioink loaded with both growth factors and implanted on a rat calvarial defect model reveals significantly improved bone regenerative effects. The TNC bioink system is a promising next-generation bioink platform because its mechanical properties can be tuned easily for high-resolution 3D bioprinting with long-term stability and its growth-factor holding capability has strong clinical applicability.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Nanocomposites
/
Bioprinting
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Small
Journal subject:
ENGENHARIA BIOMEDICA
Year:
2023
Type:
Article