Comparison of proteins with anti-influenza virus effects in parotid and submandibular-sublingual saliva in humans.
BMC Oral Health
; 22(1): 639, 2022 12 24.
Article
in En
| MEDLINE
| ID: mdl-36566172
ABSTRACT
BACKGROUND:
Saliva possesses antiviral activity, with submandibular-sublingual (SMSL) saliva having higher antiviral activity than parotid saliva. Various salivary proteins have inactivating effects on influenza A virus (IAV), but the detailed relationship between antiviral proteins and salivary anti-IAV activities in the parotid and SMSL glands is unknown. Here, to identify salivary proteins with anti-IAV activity, salivary proteins from parotid and SMSL glands were identified, quantified, and compared using liquid chromatography-mass spectrometry.METHODS:
Twelve healthy male volunteers participated in the study. Parotid and SMSL saliva was collected by suction and collection devices. We assessed anti-IAV activities, protein concentrations, and protein-bound sialic acid concentrations in parotid and SMSL saliva.RESULTS:
SMSL had significantly higher anti-IAV activity than parotid saliva. SMSL also had higher concentrations of glycoproteins, such as mucin 5B and mucin 7, protein-bound sialic acid, cystatins, and lysozyme C, compared with parotid saliva. Salivary mucin 5B and mucin 7 concentrations significantly positively correlated with the salivary protein-bound sialic acid concentration. Salivary anti-IAV activity significantly positively correlated with protein-bound sialic acid, mucin 5B, mucin 7, cystatin-C, -S, and -SN concentrations.CONCLUSION:
Salivary mucins, cystatins, and lysozyme C contribute to the high anti-IAV activity of SMSL saliva.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Antiviral Agents
/
Saliva
/
Salivary Proteins and Peptides
/
Alphainfluenzavirus
/
Mucin-5B
Limits:
Humans
/
Male
Language:
En
Journal:
BMC Oral Health
Journal subject:
ODONTOLOGIA
Year:
2022
Type:
Article
Affiliation country:
Japan