Measuring ventral nerve cord stiffness in live flat-dissected Drosophila embryos by atomic force microscopy.
STAR Protoc
; 3(4): 101901, 2022 12 16.
Article
in En
| MEDLINE
| ID: mdl-36595903
ABSTRACT
Drosophila is an amenable system for addressing the mechanics of morphogenesis. We describe a workflow for characterizing the mechanical properties of its ventral nerve cord (VNC), at different developmental stages, in live, flat-dissected embryos employing atomic force microscopy (AFM). AFM is performed with spherical probes, and stiffness (Young's modulus) is calculated by fitting force curves with Hertz's contact model. For complete details on the use and execution of this protocol, please refer to Karkali et al. (2022).
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1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Drosophila
Limits:
Animals
Language:
En
Journal:
STAR Protoc
Year:
2022
Type:
Article