Sperm mRNA screening by reverse transcription-recombinase polymerase amplification (RT-RPA) assay: Decision-making for differential extraction.
Anal Biochem
; 670: 115121, 2023 06 01.
Article
in En
| MEDLINE
| ID: mdl-36972745
ABSTRACT
The presence of sperm cells is an indicator for differential extraction on sexual assault samples. In general, sperm cells are identified by microscopic analysis; however, this conventional method takes time and effort, even for trained personnel. Here, we present a reverse transcription-recombinase polymerase amplification (RT-RPA) assay targeting sperm mRNA marker (PRM1). The RT-RPA assay requires only 40 min for PRM1 detection and demonstrates a sensitivity of 0.1 µL of semen. Our results indicate that the RT-RPA assay may be a rapid, simple, and specific strategy for screening sperm cells in sexual assault samples.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Recombinases
/
Reverse Transcription
Type of study:
Diagnostic_studies
/
Screening_studies
Limits:
Humans
/
Male
Language:
En
Journal:
Anal Biochem
Year:
2023
Type:
Article