C1q is increased in cerebrospinal fluid-derived extracellular vesicles in Alzheimer's disease: A multi-cohort proteomics and immuno-assay validation study.
Alzheimers Dement
; 19(11): 4828-4840, 2023 11.
Article
in En
| MEDLINE
| ID: mdl-37023079
ABSTRACT
INTRODUCTION:
Extracellular vesicles (EVs) may propagate and modulate Alzheimer's disease (AD) pathology. We aimed to comprehensively characterize the proteome of cerebrospinal fluid (CSF) EVs to identify proteins and pathways altered in AD.METHODS:
CSF EVs were isolated by ultracentrifugation (Cohort 1) or Vn96 peptide (Cohort 2) from non-neurodegenerative controls (n = 15, 16) and AD patients (n = 22, 20, respectively). EVs were subjected to untargeted quantitative mass spectrometry-based proteomics. Results were validated by enzyme-linked immunosorbent assay (ELISA) in Cohorts 3 and 4, consisting of controls (n = 16, n = 43, (Cohort3, Cohort4)), and patients with AD (n = 24, n = 100).RESULTS:
We found > 30 differentially expressed proteins in AD CSF EVs involved in immune-regulation. Increase of C1q levels in AD compared to non-demented controls was validated by ELISA (â¼ 1.5 fold, p (Cohort 3) = 0.03, p (Cohort 4) = 0.005).DISCUSSION:
EVs may be utilized as a potential biomarker and may play a so far unprecedented role in immune-regulation in AD.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Alzheimer Disease
/
Cognitive Dysfunction
/
Extracellular Vesicles
Type of study:
Etiology_studies
Limits:
Humans
Language:
En
Journal:
Alzheimers Dement
Year:
2023
Type:
Article
Affiliation country:
Germany