Selection of an aggregation-caused quenching-based fluorescent tracer for imaging studies in nano drug delivery systems.
Nanoscale
; 15(21): 9290-9296, 2023 Jun 01.
Article
in En
| MEDLINE
| ID: mdl-37158114
ABSTRACT
In order to develop and optimize nano drug delivery systems (NDDSs), it is crucial to understand their in vivo fate. We previously found that P2 (Aza-BODIPY) and P4 (BODIPY) as aggregation-caused quenching (ACQ) probes could be used to unravel the biofate of various nanoparticles owing to their water-sensitive emission. However, previous studies also found that quenched ACQ probe aggregates showed repartition into hydrophobic physiologically relevant constituents, resulting in fluorescence re-illumination. In this paper, we screened various types of fluorophores for ACQ and their re-illumination performance and focused on Aza-BODIPY dyes. BODIPY and Aza-BODIPY dyes were identified to be advantageous over other fluorophores. Some BODIPY and Aza-BODIPY dyes were selected as potential probes with improved performance against re-illumination. The best performing probes were Aza-C7 and Aza-C8. Aza-C7-loaded PMs were found to have decreased fluorescence re-illumination properties over P2 and DiR.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Boron Compounds
/
Nanoparticle Drug Delivery System
Language:
En
Journal:
Nanoscale
Year:
2023
Type:
Article