Genomics in the long-read sequencing era.
Trends Genet
; 39(9): 649-671, 2023 09.
Article
in En
| MEDLINE
| ID: mdl-37230864
ABSTRACT
Long-read sequencing (LRS) technologies have provided extremely powerful tools to explore genomes. While in the early years these methods suffered technical limitations, they have recently made significant progress in terms of read length, throughput, and accuracy and bioinformatics tools have strongly improved. Here, we aim to review the current status of LRS technologies, the development of novel methods, and the impact on genomics research. We will explore the most impactful recent findings made possible by these technologies focusing on high-resolution sequencing of genomes and transcriptomes and the direct detection of DNA and RNA modifications. We will also discuss how LRS methods promise a more comprehensive understanding of human genetic variation, transcriptomics, and epigenetics for the coming years.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Genomics
/
High-Throughput Nucleotide Sequencing
Limits:
Humans
Language:
En
Journal:
Trends Genet
Journal subject:
GENETICA
Year:
2023
Type:
Article