Spatially resolved single-cell translatomics at molecular resolution.
Science
; 380(6652): eadd3067, 2023 06 30.
Article
in En
| MEDLINE
| ID: mdl-37384709
ABSTRACT
The precise control of messenger RNA (mRNA) translation is a crucial step in posttranscriptional gene regulation of cellular physiology. However, it remains a challenge to systematically study mRNA translation at the transcriptomic scale with spatial and single-cell resolution. Here, we report the development of ribosome-bound mRNA mapping (RIBOmap), a highly multiplexed three-dimensional in situ profiling method to detect cellular translatome. RIBOmap profiling of 981 genes in HeLa cells revealed cell cycle-dependent translational control and colocalized translation of functional gene modules. We mapped 5413 genes in mouse brain tissues, yielding spatially resolved single-cell translatomic profiles for 119,173 cells and revealing cell type-specific and brain region-specific translational regulation, including translation remodeling during oligodendrocyte maturation. Our method detected widespread patterns of localized translation in neuronal and glial cells in intact brain tissue networks.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Protein Biosynthesis
/
Brain
/
RNA, Messenger
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Neuroglia
/
Chromosome Mapping
/
Single-Cell Gene Expression Analysis
/
Neurons
Limits:
Animals
/
Humans
Language:
En
Journal:
Science
Year:
2023
Type:
Article
Affiliation country:
United States