Screening and identification of specific cluster miRNAs in N2a cells infected by H7N9 virus.
Virus Genes
; 59(5): 716-722, 2023 Oct.
Article
in En
| MEDLINE
| ID: mdl-37395889
ABSTRACT
This study aims to screen and identify specific cluster miRNAs of H7N9 virus-infected N2a cells and explore the possible pathogenesis of these miRNAs. The N2a cells are infected with H7N9 and H1N1 influenza viruses, and the cells are collected at 12, 24 and 48 h to extract total RNA. To sequence miRNAs and identify different virus-specific miRNAs, high-throughput sequencing technology is used. Fifteen H7N9 virus-specific cluster miRNAs are screened, and eight of them are included in the miRBase database. These cluster-specific miRNAs regulate many signaling pathways, such as the PI3K-Akt signaling pathway, the RAS signaling pathway, the cAMP signaling pathway, actin cytoskeleton regulation and cancer-related genes. The study provides a scientific basis for the pathogenesis of H7N9 avian influenza, which is regulated by miRNAs.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
MicroRNAs
/
Influenza, Human
/
Influenza A Virus, H1N1 Subtype
/
Influenza A Virus, H7N9 Subtype
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Influenza in Birds
Type of study:
Diagnostic_studies
/
Screening_studies
Limits:
Animals
/
Humans
Language:
En
Journal:
Virus Genes
Journal subject:
BIOLOGIA MOLECULAR
/
VIROLOGIA
Year:
2023
Type:
Article
Affiliation country:
China