ER-dependent membrane repair of mycobacteria-induced vacuole damage.
mBio
; 14(5): e0094323, 2023 Oct 31.
Article
in En
| MEDLINE
| ID: mdl-37676004
ABSTRACT
IMPORTANCE Tuberculosis still remains a global burden and is one of the top infectious diseases from a single pathogen. Mycobacterium tuberculosis, the causative agent, has perfected many ways to replicate and persist within its host. While mycobacteria induce vacuole damage to evade the toxic environment and eventually escape into the cytosol, the host recruits repair machineries to restore the MCV membrane. However, how lipids are delivered for membrane repair is poorly understood. Using advanced fluorescence imaging and volumetric correlative approaches, we demonstrate that this involves the recruitment of the endoplasmic reticulum (ER)-Golgi lipid transfer protein OSBP8 in the Dictyostelium discoideum/Mycobacterium marinum system. Strikingly, depletion of OSBP8 affects lysosomal function accelerating mycobacterial growth. This indicates that an ER-dependent repair pathway constitutes a host defense mechanism against intracellular pathogens such as M. tuberculosis.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Tuberculosis
/
Mycobacterium marinum
/
Dictyostelium
/
Mycobacterium tuberculosis
Type of study:
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
MBio
Year:
2023
Type:
Article
Affiliation country:
Germany