Characterization and designing of an SAM riboswitch to establish a high-throughput screening platform for SAM overproduction in Saccharomyces cerevisiae.

Fu, Xiaomeng; Zuo, Xiaoru; Zhao, Xiaomeng; Zhang, Huizhi; Zhang, Chuanbo; Lu, Wenyu

Fu, Xiaomeng; Zuo, Xiaoru; Zhao, Xiaomeng; Zhang, Huizhi; Zhang, Chuanbo; Lu, Wenyu.

Affiliation

Fu X; School of Chemical Engineering and Technology, Tianjin University, Tianjin, China.
Zuo X; School of Chemical Engineering and Technology, Tianjin University, Tianjin, China.
Zhao X; School of Chemical Engineering and Technology, Tianjin University, Tianjin, China.
Zhang H; School of Chemical Engineering and Technology, Tianjin University, Tianjin, China.
Zhang C; School of Chemical Engineering and Technology, Tianjin University, Tianjin, China.
Lu W; Frontiers Science Center for Synthetic Biology, Tianjin University, Tianjin, China.

Biotechnol Bioeng ; 120(12): 3622-3637, 2023 12.

Article in En | MEDLINE | ID: mdl-37691180

Subject(s)

Riboswitch; S-Adenosylmethionine; S-Adenosylmethionine/metabolism; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/metabolism; High-Throughput Screening Assays; Riboswitch/genetics; Fermentation

Key words

S-adenosyl- l-methionine; directed evolution; high-throughput screening; metabolic engineering; riboswitch; semirational enzyme design

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: S-Adenosylmethionine / Riboswitch Type of study: Diagnostic_studies / Screening_studies Language: En Journal: Biotechnol Bioeng Year: 2023 Type: Article Affiliation country: China

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