Genetic engineering strategy for generating a stable dsRNA virus vector using a virus-like codon-modified transgene.
J Virol
; 97(10): e0049223, 2023 10 31.
Article
in En
| MEDLINE
| ID: mdl-37732784
ABSTRACT
IMPORTANCE The stabilities of transgenes in RNA virus vectors differ between the genes of interest, but the molecular mechanisms determining genetic stability remain unknown. This study demonstrated that the stability of a transgene was affected by the nucleotide composition, and altering the codon usage of transgenes to resemble that of the viral genome significantly increased transgene stability in double-stranded RNA virus vectors. The virus-like codon modification strategy enabled generation of stable rotavirus and mammalian orthoreovirus vectors, which could be developed as machinery for gene delivery to the intestines and/or respiratory organs. This technology has further potential to be expanded to other RNA viruses.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
RNA Viruses
/
Double Stranded RNA Viruses
Limits:
Animals
Language:
En
Journal:
J Virol
Year:
2023
Type:
Article
Affiliation country:
Japan