Alphavirus-based replicons demonstrate different interactions with host cells and can be optimized to increase protein expression.
J Virol
; 97(11): e0122523, 2023 Nov 30.
Article
in En
| MEDLINE
| ID: mdl-37877718
ABSTRACT
IMPORTANCE Alphavirus replicons are being developed as self-amplifying RNAs aimed at improving the efficacy of mRNA vaccines. These replicons are convenient for genetic manipulations and can express heterologous genetic information more efficiently and for a longer time than standard mRNAs. However, replicons mimic many aspects of viral replication in terms of induction of innate immune response, modification of cellular transcription and translation, and expression of nonstructural viral genes. Moreover, all replicons used in this study demonstrated expression of heterologous genes in cell- and replicon's origin-specific modes. Thus, many aspects of the interactions between replicons and the host remain insufficiently investigated, and further studies are needed to understand the biology of the replicons and their applicability for designing a new generation of mRNA vaccines. On the other hand, our data show that replicons are very flexible expression systems, and additional modifications may have strong positive impacts on protein expression.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Replicon
/
Viral Proteins
/
Gene Expression Regulation, Viral
/
Alphavirus
/
Host Microbial Interactions
Language:
En
Journal:
J Virol
Year:
2023
Type:
Article
Affiliation country:
United States