Cell Size Contributes to Single-Cell Proteome Variation.
J Proteome Res
; 22(12): 3773-3779, 2023 12 01.
Article
in En
| MEDLINE
| ID: mdl-37910793
ABSTRACT
Accurate measurements of the molecular composition of single cells will be necessary for understanding the relationship between gene expression and function in diverse cell types. One of the most important phenotypes that differs between cells is their size, which was recently shown to be an important determinant of proteome composition in populations of similarly sized cells. We, therefore, sought to test if the effects of the cell size on protein concentrations were also evident in single-cell proteomics data. Using the relative concentrations of a set of reference proteins to estimate a cell's DNA-to-cell volume ratio, we found that differences in the cell size explain a significant amount of cell-to-cell variance in two published single-cell proteome data sets.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Proteome
Language:
En
Journal:
J Proteome Res
Journal subject:
BIOQUIMICA
Year:
2023
Type:
Article
Affiliation country:
United States