Whole blood stimulation as a tool for studying the human immune system.
Eur J Immunol
; 54(2): e2350519, 2024 Feb.
Article
in En
| MEDLINE
| ID: mdl-38103010
ABSTRACT
The human immune system is best accessible via tissues and organs not requiring major surgical intervention, such as blood. In many circumstances, circulating immune cells correlate with an individual's health state and give insight into physiological and pathophysiological processes. Stimulating whole blood ex vivo is a powerful tool to investigate immune responses. In the context of clinical research, the applications of whole blood stimulation include host immunity, disease characterization, diagnosis, treatment, and drug development. Here, we summarize different setups and readouts of whole blood assays and discuss applications for preclinical research and clinical practice. Finally, we propose combining whole blood stimulation with high-throughput technologies, such as single-cell RNA-sequencing, to comprehensively analyze the human immune system for the identification of biomarkers, therapeutic interventions as well as companion diagnostics.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Blood
/
Immune System
Limits:
Humans
Language:
En
Journal:
Eur J Immunol
Year:
2024
Type:
Article
Affiliation country:
Germany