Integrated Advanced Molecular Tools Predict In Situ cVOC Degradation Rates: Field Demonstration.
Environ Sci Technol
; 58(1): 557-569, 2024 Jan 09.
Article
in En
| MEDLINE
| ID: mdl-38109066
ABSTRACT
Chlorinated volatile organic compound (cVOC) degradation rate constants are crucial information for site management. Conventional approaches generate rate estimates from the monitoring and modeling of cVOC concentrations. This requires time series data collected along the flow path of the plume. The estimates of rate constants are often plagued by confounding issues, making predictions cumbersome and unreliable. Laboratory data suggest that targeted quantitative analysis of Dehalococcoides mccartyi (Dhc) biomarker genes (qPCR) and proteins (qProt) can be directly correlated with reductive dechlorination activity. To assess the potential of qPCR and qProt measurements to predict rates, we collected data from cVOC-contaminated aquifers. At the benchmark study site, the rate constant for degradation of cis-dichloroethene (cDCE) extracted from monitoring data was 11.0 ± 3.4 yr-1, and the rate constant predicted from the abundance of TceA peptides was 6.9 yr-1. The rate constant for degradation of vinyl chloride (VC) from monitoring data was 8.4 ± 5.7 yr-1, and the rate constant predicted from the abundance of TceA peptides was 5.2 yr-1. At the other study sites, the rate constants for cDCE degradation predicted from qPCR and qProt measurements agreed within a factor of 4. Under the right circumstances, qPCR and qProt measurements can be useful to rapidly predict rates of cDCE and VC biodegradation, providing a major advance in effective site management.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Trichloroethylene
/
Vinyl Chloride
/
Chloroflexi
Language:
En
Journal:
Environ Sci Technol
Year:
2024
Type:
Article
Affiliation country:
United States