Rapid On-Site Detection of Arboviruses by a Direct RT-qPCR Assay.
Biosensors (Basel)
; 13(12)2023 Dec 16.
Article
in En
| MEDLINE
| ID: mdl-38131795
ABSTRACT
Arthropod-borne diseases currently constitute a source of major health concerns worldwide. They account for about 50% of global infectious diseases and cause nearly 700,000 deaths every year. Their rapid increase and spread constitute a huge challenge for public health, highlighting the need for early detection during epidemics, to curtail the virus spread, and to enhance outbreak management. Here, we compared a standard quantitative polymerase chain reaction (RT-qPCR) and a direct RT-qPCR assay for the detection of Zika (ZIKV), Chikungunya (CHIKV), and Rift Valley Fever (RVFV) viruses from experimentally infected-mosquitoes. The direct RT-qPCR could be completed within 1.5 h and required 1 µL of viral supernatant from homogenized mosquito body pools. Results showed that the direct RT-qPCR can detect 85.71%, 89%, and 100% of CHIKV, RVFV, and ZIKV samples by direct amplifications compared to the standard method. The use of 110 diluted supernatant is suggested for CHIKV and RVFV direct RT-qPCR. Despite a slight drop in sensitivity for direct PCR, our technique is more affordable, less time-consuming, and provides a better option for qualitative field diagnosis during outbreak management. It represents an alternative when extraction and purification steps are not possible because of insufficient sample volume or biosecurity issues.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Arboviruses
/
Chikungunya virus
/
Dengue Virus
/
Chikungunya Fever
/
Zika Virus
/
Zika Virus Infection
/
Culicidae
Limits:
Animals
Language:
En
Journal:
Biosensors (Basel)
Year:
2023
Type:
Article
Affiliation country:
Senegal