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DNA methylation remodeled amino acids biosynthesis regulates flower senescence in carnation (Dianthus caryophyllus).
Feng, Shan; Jiang, Xinyu; Huang, Zhiheng; Li, Fan; Wang, Ruiming; Yuan, Xinyi; Sun, Zheng; Tan, Hualiang; Zhong, Linlin; Li, Shenchong; Cheng, Yunjiang; Bao, Manzhu; Qiao, Hong; Song, Qingxin; Wang, Jihua; Zhang, Fan.
Affiliation
  • Feng S; National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Huazhong Agricultural University, Wuhan, 430070, China.
  • Jiang X; Hubei Hongshan Laboratory, Wuhan, 430070, China.
  • Huang Z; National R&D Center for Citrus Postharvest Technology, Huazhong Agricultural University, Wuhan, 430070, China.
  • Li F; State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China.
  • Wang R; National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Huazhong Agricultural University, Wuhan, 430070, China.
  • Yuan X; Hubei Hongshan Laboratory, Wuhan, 430070, China.
  • Sun Z; National R&D Center for Citrus Postharvest Technology, Huazhong Agricultural University, Wuhan, 430070, China.
  • Tan H; Yunnan Seed Laboratory, Kunming, 650200, China.
  • Zhong L; Floriculture Research Institute, Yunnan Academy of Agricultural Sciences, National Engineering Research Center for Ornamental Horticulture, Key Laboratory for Flower Breeding of Yunnan Province, Kunming, 650200, China.
  • Li S; National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Huazhong Agricultural University, Wuhan, 430070, China.
  • Cheng Y; Hubei Hongshan Laboratory, Wuhan, 430070, China.
  • Bao M; National R&D Center for Citrus Postharvest Technology, Huazhong Agricultural University, Wuhan, 430070, China.
  • Qiao H; National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Huazhong Agricultural University, Wuhan, 430070, China.
  • Song Q; Hubei Hongshan Laboratory, Wuhan, 430070, China.
  • Wang J; National R&D Center for Citrus Postharvest Technology, Huazhong Agricultural University, Wuhan, 430070, China.
  • Zhang F; National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Huazhong Agricultural University, Wuhan, 430070, China.
New Phytol ; 241(4): 1605-1620, 2024 Feb.
Article in En | MEDLINE | ID: mdl-38179647
ABSTRACT
Dynamic DNA methylation regulatory networks are involved in many biological processes. However, how DNA methylation patterns change during flower senescence and their relevance with gene expression and related molecular mechanism remain largely unknown. Here, we used whole genome bisulfite sequencing to reveal a significant increase of DNA methylation in the promoter region of genes during natural and ethylene-induced flower senescence in carnation (Dianthus caryophyllus L.), which was correlated with decreased expression of DNA demethylase gene DcROS1. Silencing of DcROS1 accelerated while overexpression of DcROS1 delayed carnation flower senescence. Moreover, among the hypermethylated differentially expressed genes during flower senescence, we identified two amino acid biosynthesis genes, DcCARA and DcDHAD, with increased DNA methylation and reduced expression in DcROS1 silenced petals, and decreased DNA methylation and increased expression in DcROS1 overexpression petals, accompanied by decreased or increased amino acids content. Silencing of DcCARA and DcDHAD accelerates carnation flower senescence. We further showed that adding corresponding amino acids could largely rescue the senescence phenotype of DcROS1, DcCARA and DcDHAD silenced plants. Our study not only demonstrates an essential role of DcROS1-mediated remodeling of DNA methylation in flower senescence but also unravels a novel epigenetic regulatory mechanism underlying DNA methylation and amino acid biosynthesis during flower senescence.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Dianthus / Syzygium Language: En Journal: New Phytol Journal subject: BOTANICA Year: 2024 Type: Article Affiliation country: China

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Dianthus / Syzygium Language: En Journal: New Phytol Journal subject: BOTANICA Year: 2024 Type: Article Affiliation country: China