Co-expression of IL-21-Enhanced NKG2D CAR-NK cell therapy for lung cancer.
BMC Cancer
; 24(1): 119, 2024 Jan 23.
Article
in En
| MEDLINE
| ID: mdl-38263004
ABSTRACT
BACKGROUND:
Adoptive cell therapy has achieved great success in treating hematological malignancies. However, the production of chimeric antigen receptor T (CAR-T) cell therapy still faces various difficulties. Natural killer (NK)-92 is a continuously expandable cell line and provides a promising alternative for patient's own immune cells.METHODS:
We established CAR-NK cells by co-expressing natural killer group 2 member D (NKG2D) and IL-21, and evaluated the efficacy of NKG2D-IL-21 CAR-NK cells in treating lung cancer in vitro and in vivo.RESULTS:
Our data suggested that the expression of IL-21 effectively increased the cytotoxicity of NKG2D CAR-NK cells against lung cancer cells in a dose-dependent manner and suppressed tumor growth in vitro and in vivo. In addition, the proliferation of NKG2D-IL-21 CAR-NK cells were enhanced while the apoptosis and exhaustion of these cells were suppressed. Mechanistically, IL-21-mediated NKG2D CAR-NK cells function by activating AKT signaling pathway.CONCLUSION:
Our findings provide a novel option for treating lung cancer using NKG2D-IL-21 CAR-NK cell therapy.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Interleukins
/
NK Cell Lectin-Like Receptor Subfamily K
/
Lung Neoplasms
Limits:
Humans
Language:
En
Journal:
BMC Cancer
Journal subject:
NEOPLASIAS
Year:
2024
Type:
Article
Affiliation country:
China