The role of vasoactive intestinal peptide (VIP) in atropine-related inhibition of the progression of myopia.
BMC Ophthalmol
; 24(1): 41, 2024 Jan 26.
Article
in En
| MEDLINE
| ID: mdl-38279089
ABSTRACT
OBJECTIVE:
This study aimed to investigate the potential involvement of vasoactive intestinal polypeptide (VIP) in myopia development and its contribution to the mechanism of action of the anti-myopia drug, atropine.METHODS:
Thirty-three-week-old guinea pigs were randomly divided into normal control (NC, n = 10), monocularly form-deprived (FDM, n = 10), and FDM treated with 1% atropine (FDM + AT, n = 10) groups. The diopter and axial length were measured at 0, 2, and 4 weeks. Guinea pig eyeballs were removed at week four, fixed, and stained for morphological changes. Immunohistochemistry (IHC) and in situ hybridization (ISH) were performed to evaluate VIP protein and mRNA levels.RESULTS:
The FDM group showed an apparent myopic shift compared to the control group. The results of the H&E staining were as follows the cells of the inner/outer nuclear layers and retinal ganglion cells were disorganized; the choroidal thickness (ChT), blood vessel lumen, and area were decreased; the sclera was thinner, with disordered fibers and increased interfibrillar space. IHC and ISH revealed that VIP's mRNA and protein expressions were significantly up-regulated in the retina of the FDM group. Atropine treatment attenuated FDM-induced myopic shift and fundus changes, considerably reducing VIP's mRNA and protein expressions.CONCLUSIONS:
The findings of elevated VIP mRNA and protein levels observed in the FDM group indicate the potential involvement of VIP in the pathogenesis and progression of myopia. The ability of atropine to reduce this phenomenon suggests that this may be one of the molecular mechanisms for atropine to control myopia.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Vasoactive Intestinal Peptide
/
Myopia
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
BMC Ophthalmol
Journal subject:
OFTALMOLOGIA
Year:
2024
Type:
Article
Affiliation country:
China