Your browser doesn't support javascript.
loading
Efficient production and characterization of melanin from Thermothelomyces hinnuleus SP1, isolated from the coal mines of Chhattisgarh, India.
Pandey, Shalini; Meshram, Vineet; Yehia, Hany M; Alzahrani, Abdulhakeem; Akhtar, Nadeem; Sur, Arunima.
Affiliation
  • Pandey S; Amity Institute of Biotechnology, Amity University, Raipur, Chhattisgarh, India.
  • Meshram V; Department of Biotechnology and Microbiology, Anjaneya University, Raipur, Chhattisgarh, India.
  • Yehia HM; Department of Food Science and Nutrition, Faculty of Home Economics, Helwan University, Cairo, Egypt.
  • Alzahrani A; Department of Food Science and Nutrition, College of Food and Agricultural Sciences, King Saud University, Riyadh, Saudi Arabia.
  • Akhtar N; Department of Animal Biosciences, University of Guelph, Guelph, ON, Canada.
  • Sur A; Amity Institute of Biotechnology, Amity University, Raipur, Chhattisgarh, India.
Front Microbiol ; 14: 1320116, 2023.
Article in En | MEDLINE | ID: mdl-38293558
ABSTRACT
In the present study, fungi were isolated and screened from barren land in south-eastern Coalfields limited (SECL) in Chhattisgarh, India. Out of 14 isolated fungi, only three fungal isolates exhibited pigmentation in screening studies. The isolated fungal strain SP1 exhibited the highest pigmentation, which was further utilized for in vivo production, purification, and characterization of melanin pigment. The physical and chemical properties of the fungal pigment showed insolubility in organic solvents and water, solubility in alkali, precipitation in acid, and decolorization with oxidizing agents. The physiochemical characterization and analytical studies of the extracted pigment using ultraviolet-visible spectroscopy and Fourier transform infrared (FTIR) confirmed it as a melanin pigment. The melanin-producing fungus SP1 was identified as Thermothelomyces hinnuleus based on 18S-rRNA sequence analysis. Furthermore, to enhance melanin production, a response surface methodology (RSM) was employed, specifically utilizing the central composite design (CCD). This approach focused on selecting efficient growth as well as progressive yield parameters such as optimal temperature (34.4°C), pH (5.0), and trace element concentration (56.24 mg). By implementing the suggested optimal conditions, the production rate of melanin increased by 62%, resulting in a yield of 28.3 mg/100 mL, which is comparatively higher than the actual yield (17.48 ± 2.19 mg/100 mL). Thus, T. hinnuleus SP1 holds great promise as a newly isolated fungal strain that could be used for the industrial production of melanin.
Key words

Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: Front Microbiol Year: 2023 Type: Article Affiliation country: India

Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: Front Microbiol Year: 2023 Type: Article Affiliation country: India