Landscape of RNA pseudouridylation in archaeon Sulfolobus islandicus.
Nucleic Acids Res
; 52(8): 4644-4658, 2024 May 08.
Article
in En
| MEDLINE
| ID: mdl-38375885
ABSTRACT
Pseudouridine, one of the most abundant RNA modifications, is synthesized by stand-alone or RNA-guided pseudouridine synthases. Here, we comprehensively mapped pseudouridines in rRNAs, tRNAs and small RNAs in the archaeon Sulfolobus islandicus and identified Cbf5-associated H/ACA RNAs. Through genetic deletion and in vitro modification assays, we determined the responsible enzymes for these modifications. The pseudouridylation machinery in S. islandicus consists of the stand-alone enzymes aPus7 and aPus10, and six H/ACA RNA-guided enzymes that account for all identified pseudouridines. These H/ACA RNAs guide the modification of all eleven sites in rRNAs, two sites in tRNAs, and two sites in CRISPR RNAs. One H/ACA RNA shows exceptional versatility by targeting eight different sites. aPus7 and aPus10 are responsible for modifying positions 13, 54 and 55 in tRNAs. We identified four atypical H/ACA RNAs that lack the lower stem and the ACA motif and confirmed their function both in vivo and in vitro. Intriguingly, atypical H/ACA RNAs can be modified by Cbf5 in a guide-independent manner. Our data provide the first global view of pseudouridylation in archaea and reveal unexpected structures, substrates, and activities of archaeal H/ACA RNPs.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Pseudouridine
/
RNA, Transfer
/
Sulfolobus
/
RNA, Archaeal
Language:
En
Journal:
Nucleic Acids Res
Year:
2024
Type:
Article
Affiliation country:
China