Autophagy activity is increased in the cumulus cells of women with poor ovarian response.
Taiwan J Obstet Gynecol
; 63(2): 205-213, 2024 Mar.
Article
in En
| MEDLINE
| ID: mdl-38485316
ABSTRACT
OBJECTIVE:
To evaluate the autophagy status of cumulus cells (CCs) in women with poor ovarian response (POR). MATERIALS ANDMETHODS:
CCs were divided into normal ovarian response (NOR) group and POR group. The ultrastructure of autophagy was analyzed by transmission electron microscopy (NOR n = 18, POR n = 26). The mRNA and protein of autophagy markers were detected by Quantitative real-time polymerase chain reaction (NOR n = 15, POR n = 19) and Western blotting (NOR n = 41, POR n = 38), respectively.RESULTS:
Transmission electron microscopy demonstrated abundant autophagosomes and even autophagic death in the POR group. There were no differences in LC3 and P62 mRNA expression between the two groups (p > 0.05). The BCL2 mRNA expression was lower in the POR group (p < 0.05). Moreover, the LC3 II/I ratio and the P62 protein expression were significantly higher in the POR group (p < 0.05).CONCLUSIONS:
Autophagy in CCs of POR women is activated and the autophagic flux is blocked. The up-regulation of autophagy in CCs may be related to the pathogenesis of POR.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Autophagy
/
Cumulus Cells
Limits:
Female
/
Humans
Language:
En
Journal:
Taiwan J Obstet Gynecol
/
Taiwan. j. obstet. gynecol
/
Taiwanese journal of obstetrics & gynecology
Journal subject:
GINECOLOGIA
/
OBSTETRICIA
Year:
2024
Type:
Article