The effect of ionic redistributions on the microwave dielectric response of cytosol water upon glucose uptake.
Eur Biophys J
; 53(4): 183-192, 2024 May.
Article
in En
| MEDLINE
| ID: mdl-38647542
ABSTRACT
The sensitivity of cytosol water's microwave dielectric (MD) response to D-glucose uptake in Red Blood Cells (RBCs) allows the detailed study of cellular mechanisms as a function of controlled exposures to glucose and other related analytes like electrolytes. However, the underlying mechanism behind the sensitivity to glucose exposure remains a topic of debate. In this research, we utilize MDS within the frequency range of 0.5-40 GHz to explore how ionic redistributions within the cell impact the microwave dielectric characteristics associated with D-glucose uptake in RBC suspensions. Specifically, we compare glucose uptake in RBCs exposed to the physiological concentration of Ca2+ vs. Ca-free conditions. We also investigate the potential involvement of Na+/K+ redistribution in glucose-mediated dielectric response by studying RBCs treated with a specific Na+/K+ pump inhibitor, ouabain. We present some insights into the MD response of cytosol water when exposed to Ca2+ in the absence of D-glucose. The findings from this study confirm that ion-induced alterations in bound/bulk water balance do not affect the MD response of cytosol water during glucose uptake.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Water
/
Cytosol
/
Erythrocytes
/
Glucose
/
Microwaves
Limits:
Humans
Language:
En
Journal:
Eur Biophys J
Journal subject:
BIOFISICA
Year:
2024
Type:
Article
Affiliation country:
Israel