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Species Difference? Bovine, Trout, and Human Plasma Protein Binding of Per- and Polyfluoroalkyl Substances.
Qin, Weiping; Escher, Beate I; Huchthausen, Julia; Fu, Qiuguo; Henneberger, Luise.
Affiliation
  • Qin W; Department of Cell Toxicology, UFZ─Helmholtz Centre for Environmental Research, 04318 Leipzig, Germany.
  • Escher BI; Environmental Toxicology, Department of Geosciences, Eberhard Karls University Tübingen, Schnarrenbergstr. 94-96, DE-72076 Tübingen, Germany.
  • Huchthausen J; Department of Cell Toxicology, UFZ─Helmholtz Centre for Environmental Research, 04318 Leipzig, Germany.
  • Fu Q; Environmental Toxicology, Department of Geosciences, Eberhard Karls University Tübingen, Schnarrenbergstr. 94-96, DE-72076 Tübingen, Germany.
  • Henneberger L; Department of Cell Toxicology, UFZ─Helmholtz Centre for Environmental Research, 04318 Leipzig, Germany.
Environ Sci Technol ; 58(23): 9954-9966, 2024 Jun 11.
Article in En | MEDLINE | ID: mdl-38804966
ABSTRACT
Per- and polyfluoroalkyl substances (PFAS) strongly bind to proteins and lipids in blood, which govern their accumulation and distribution in organisms. Understanding the plasma binding mechanism and species differences will facilitate the quantitative in vitro-to-in vivo extrapolation and improve risk assessment of PFAS. We studied the binding mechanism of 16 PFAS to bovine serum albumin (BSA), trout, and human plasma using solid-phase microextraction. Binding of anionic PFAS to BSA and human plasma was found to be highly concentration-dependent, while trout plasma binding was linear for the majority of the tested PFAS. At a molar ratio of PFAS to protein ν < 0.1 molPFAS/molprotein, the specific protein binding of anionic PFAS dominated their human plasma binding. This would be the scenario for physiological conditions (ν < 0.01), whereas in in vitro assays, PFAS are often dosed in excess (ν > 1) and nonspecific binding becomes dominant. BSA was shown to serve as a good surrogate for human plasma. As trout plasma contains more lipids, the nonspecific binding to lipids affected the affinities of PFAS for trout plasma. Mass balance models that are parameterized with the protein-water and lipid-water partitioning constants (chemical characteristics), as well as the protein and lipid contents of the plasma (species characteristics), were successfully used to predict the binding to human and trout plasma.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Protein Binding / Trout / Blood Proteins / Fluorocarbons Limits: Animals / Humans Language: En Journal: Environ Sci Technol Year: 2024 Type: Article Affiliation country: Germany

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Protein Binding / Trout / Blood Proteins / Fluorocarbons Limits: Animals / Humans Language: En Journal: Environ Sci Technol Year: 2024 Type: Article Affiliation country: Germany