The growth factors amphiregulin and epiregulin are novel stimulators of feline ovarian granulosa cell functions.
Reprod Domest Anim
; 59(6): e14628, 2024 Jun.
Article
in En
| MEDLINE
| ID: mdl-38828525
ABSTRACT
This study aimed to investigate the impact of the epidermal growth factor receptor ligands amphiregulin (AREG) and epiregulin (EREG) on the fundamental functions of feline ovarian granulosa cells. Granulosa cells isolated from feline ovaries were incubated with AREG and EREG (0, 0.1, 1 or 10 ng/mL). The effects of these growth factors on cell viability, proliferation (assessed through BrdU incorporation), nuclear apoptosis (evaluated through nuclear DNA fragmentation) and the release of progesterone and estradiol were determined using Cell Counting Kit-8 assays, BrdU analysis, TUNEL assays and ELISAs, respectively. Both AREG and EREG increased cell viability, proliferation and steroid hormone release and reduced apoptosis. The present findings suggest that these epidermal growth factor receptor ligands may serve as physiological stimulators of feline ovarian cell functions.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Apoptosis
/
Amphiregulin
/
Epiregulin
/
Granulosa Cells
Limits:
Animals
Language:
En
Journal:
Reprod Domest Anim
Journal subject:
MEDICINA REPRODUTIVA
/
MEDICINA VETERINARIA
Year:
2024
Type:
Article
Affiliation country:
Slovakia