Construction of multilayered small intestine-like tissue by reproducing interstitial flow.
Cell Stem Cell
; 31(9): 1315-1326.e8, 2024 Sep 05.
Article
in En
| MEDLINE
| ID: mdl-38996472
ABSTRACT
Recent advances have made modeling human small intestines in vitro possible, but it remains a challenge to recapitulate fully their structural and functional characteristics. We suspected interstitial flow within the intestine, powered by circulating blood plasma during embryonic organogenesis, to be a vital factor. We aimed to construct an in vivo-like multilayered small intestinal tissue by incorporating interstitial flow into the system and, in turn, developed the micro-small intestine system by differentiating definitive endoderm and mesoderm cells from human pluripotent stem cells simultaneously on a microfluidic device capable of replicating interstitial flow. This approach enhanced cell maturation and led to the development of a three-dimensional small intestine-like tissue with villi-like epithelium and an aligned mesenchymal layer. Our micro-small intestine system not only overcomes the limitations of conventional intestine models but also offers a unique opportunity to gain insights into the detailed mechanisms underlying intestinal tissue development.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Tissue Engineering
/
Intestine, Small
Limits:
Humans
Language:
En
Journal:
Cell Stem Cell
/
Cell stem cell (Online)
Year:
2024
Type:
Article