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3D Bioprinted Tissue-Engineered Bone with Enhanced Mechanical Strength and Bioactivities: Accelerating Bone Defect Repair through Sequential Immunomodulatory Properties.
Liu, Daqian; Liu, Jingsong; Zhao, Pengcheng; Peng, Zhibin; Geng, Zhibin; Zhang, Jingwei; Zhang, Zhuoran; Shen, Ruifang; Li, Xiang; Wang, Xiaoyu; Li, Shuangzuo; Wang, Jiankai; Wang, Xintao.
Affiliation
  • Liu D; Department of Orthopedic Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin Medical University, 246 Xuefu Road, Harbin, 150001, P. R. China.
  • Liu J; Key Laboratory of Myocardial Ischemia, Ministry of Education, Harbin Medical University, 148 Baojian Road, Harbin, 150001, P. R. China.
  • Zhao P; Department of Orthopedic Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin Medical University, 199 Dazhi Street, Harbin, 150001, P. R. China.
  • Peng Z; Key Laboratory of 3D Printing Process and Equipment of Zhejiang Province, School of Mechanical Engineering, Zhejiang University, 38 Zheda Road, Hangzhou, 310027, P. R. China.
  • Geng Z; Center for Endemic Disease Control, Chinese Center for Disease Control and Prevention, Harbin Medical University, 157 Baojian Road, Harbin, 150081, P. R. China.
  • Zhang J; Key Laboratory of Etiology and Epidemiology, Education Bureau of Heilongjiang Province and Ministry of Health, Harbin Medical University, 157 Baojian Road, Harbin, 150081, P. R. China.
  • Zhang Z; Department of Orthopedic Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin Medical University, 246 Xuefu Road, Harbin, 150001, P. R. China.
  • Shen R; Key Laboratory of Myocardial Ischemia, Ministry of Education, Harbin Medical University, 148 Baojian Road, Harbin, 150001, P. R. China.
  • Li X; Department of Orthopedic Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin Medical University, 246 Xuefu Road, Harbin, 150001, P. R. China.
  • Wang X; Key Laboratory of Myocardial Ischemia, Ministry of Education, Harbin Medical University, 148 Baojian Road, Harbin, 150001, P. R. China.
  • Li S; Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, Zhejiang University of Technology, 18 Chaowang Road, Hangzhou, 310014, P. R. China.
  • Wang J; Laboratory for Space Environment and Physical Sciences, Harbin Institute of Technology, 92 Xidazhi Street, Harbin, 150001, P. R. China.
  • Wang X; State Key Laboratory of Mechanical System and Vibration, School of Mechanical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, P. R. China.
Adv Healthc Mater ; : e2401919, 2024 Aug 18.
Article in En | MEDLINE | ID: mdl-39155410
ABSTRACT
In this study, a new-generation tissue-engineered bone capable of temporally regulating the immune response, balancing proinflammatory and anti-inflammatory activities, and facilitating bone regeneration and repair to address the challenges of delayed healing and nonunion in large-sized bone defects, is innovatively developed. Using the innovative techniques including multiphysics-assisted combined decellularization, side-chain biochemical modification, and sterile freeze-drying, a novel photocurable extracellular matrix hydrogel, methacrylated bone-derived decellularized extracellular matrix (bdECM-MA), is synthesized. After incorporating the bdECM-MA with silicon-substituted calcium phosphate and bone marrow mesenchymal stem cells, the tissue-engineered bone is fabricated through digital light processing 3D bioprinting. This study provides in vitro confirmation that the engineered bone maintains high cellular viability while achieving MPa-level mechanical strength. Moreover, this engineered bone exhibits excellent osteogenesis, angiogenesis, and immunomodulatory functions. One of the molecular mechanisms of the immunomodulatory function involves the inhibition of the p38-MAPK pathway. A pioneering in vivo discovery is that the natural biomaterial-based tissue-engineered bone demonstrates sequential immunomodulatory properties that activate proinflammatory and anti-inflammatory responses in succession, significantly accelerating the repair of bone defects. This study provides a new research basis and an effective method for developing autogenous bone substitute materials and treating large-sized bone defects.
Key words

Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: Adv Healthc Mater Year: 2024 Type: Article

Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: Adv Healthc Mater Year: 2024 Type: Article