Environmental pH modulation of collagenase in normal human fibroblast cultures.
Biochim Biophys Acta
; 585(3): 389-97, 1979 Jul 04.
Article
in En
| MEDLINE
| ID: mdl-39627
ABSTRACT
Normal human skin fibroblast cultures have been used to assess the effects of relatively minor changes in environmental pH on collagenase, a major extracellular gene product. Collagenase accumulation in the culture medium, measured both as enzyme activity and immunoreactive material, was 2- to 10-fold greater at pH 7.6--8.2 than at pH 6.8--7.2. The pH-associated increase in collagenase was paralleled by an increase in general protein synthesis. Nevertheless, prototypic lysosomal and cytoplasmic enzymes changed very little under identical culturing conditions. Although substantial intracellular protein degradation occurred at all pH values, the small differences either in general protein degradation or in specific collagenase degradation in the medium were of insufficient magnitude to account for the increased accumulation of collagenase.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Microbial Collagenase
/
Fibroblasts
Limits:
Humans
Language:
En
Journal:
Biochim Biophys Acta
Year:
1979
Type:
Article