[Isolation and characterization of pregnancy associated alpha2 glycoprotein]. / Isolierung und Charakterisierung des schwangerschafts-assoziierten alpha2-Glykoproteins (alpha2-PAG)
Blut
; 33(6): 377-88, 1976 Dec.
Article
in De
| MEDLINE
| ID: mdl-63301
ABSTRACT
A method using immunoadsorbents for the isolation of pregnancy-associated alpha2-glycoprotein (alpha2-PAG) from the extract of human placentae is described. The physical properties and the chemical composition of the purified protein are determined alpha2PAG sediments with 11,5 S, has a molecular weight of 360 000 daltons and is composed of subunits having a molecular weight of 180000, which are held together by disulfide bonds. The isoelectric point was found to be pH 4,7 and the extinction coefficient (E1%1cm) was determined to be 9,7 at 277 nm. The carbohydrate content of the molecule amounts to 12,1% (hexose 6,0%, hexosamine 3,7%, fucose 0,06%, sialic acid 2,4%). An analysis of the amino acids is reported, too. The purified alpha2PAG was used to determine the absolute concentrations of this protein in a reference standard and in sera.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Alpha-Macroglobulins
/
Pregnancy
Type of study:
Risk_factors_studies
Limits:
Female
/
Humans
Language:
De
Journal:
Blut
Year:
1976
Type:
Article