Inhibin-like activity in extracts of rabbit placentae.
Placenta
; 2(3): 259-64, 1981.
Article
in En
| MEDLINE
| ID: mdl-6792621
ABSTRACT
Following the demonstration of inhibin activity in the ovary, a search was conducted for such activity in the rabbit placenta. A bioassay was utilized that consisted of 16 hours' incubation of the test substance with cultured rat anterior pituitary cells and determination of gonadotrophins in the recovered media. A crude extract of rabbit placenta had no effect on luteinizing hormone (LH) but a slight and insignificant inhibiting effect on follicle-stimulating hormone (FSH) secretion. Ethanol-extracted low steroid preparation of the rabbit placenta significantly inhibited FSH, but had no effect on LH secretion. On Sephadex G100 chromatography a fraction was identified which had inhibin activity. In a parallel chromatography of human seminal fluid inhibin activity was identified in the same elution volume as that of the rabbit placenta extract. It is suggested that rabbit placenta extract contains a non-steroidal substance that interferes with pituitary secretion of FSH, and that this substance may be inhibin.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Placenta
/
Testicular Hormones
/
Proteins
/
Gonadotropin-Releasing Hormone
Limits:
Animals
/
Pregnancy
Language:
En
Journal:
Placenta
Year:
1981
Type:
Article