Clinical implications of enzyme-mediated alterations of gamma-aminobutyric acid content in human CSF.
Arch Neurol
; 38(8): 491-4, 1981 Aug.
Article
in En
| MEDLINE
| ID: mdl-7247786
ABSTRACT
Pooled samples of lumbar CSF from nine patients with neurologic disorders were aliquoted and subjected to the differential influence of temperature for four hours. The determination of the gamma-amino-butyric acid (GABA) levels in CSF by ion-exchange-fluorometric analysis before and after incubation showed a progressive increase in GABA content of CSF as a function of temperature, reaching a maximum at 50 degrees C. However, no increases in GABA level were noted in CSF incubated at 80 degrees C or 100 degrees C. These in vitro increases in the GABA content of untreated CSF appear to be entirely secondary to enzyme action, subject to individual and temperature variability, and necessitate standardization of clinical CSF protocols.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Enzymes
/
Gamma-Aminobutyric Acid
Type of study:
Guideline
Limits:
Humans
Language:
En
Journal:
Arch Neurol
Year:
1981
Type:
Article