Soluble Fas/APO-1 in tumor cells: a potential regulator of apoptosis?
Cancer Lett
; 94(1): 1-8, 1995 Jul 20.
Article
in En
| MEDLINE
| ID: mdl-7542559
ABSTRACT
Fas/APO-1, a member of the NGF/TNF receptor superfamily expressed on the cell-surface of normal and malignant cells, is known to induce cell death by apoptosis. In the present study, we have investigated Fas/APO-1 gene defects in a human osteosarcoma cell line resistant to the apoptosis-inducing effects of anti-Fas. cDNA cloning and sequencing revealed that these cells contained both 'authentic' and mutant Fas/APO-1 containing a 63 base pair in-frame deletion spanning the transmembrane domain, designated DFas/APO-1. Direct evidence for the existence of a soluble Fas/APO-1 protein was obtained by immunoprecipitation and Western blotting. Taken together with prior studies demonstrating a role for Fas/APO-1 and Fas ligand, respectively, in tumor target cell killing by cytotoxic T-lymphocytes, production of soluble Fas/APO-1 might have significant implications in malignant disease pathogenesis.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Osteosarcoma
/
Gene Deletion
/
Apoptosis
/
Antigens, Surface
Limits:
Humans
Language:
En
Journal:
Cancer Lett
Year:
1995
Type:
Article
Affiliation country:
United States