An HLA-B regulatory element binds a factor immunologically related to the upstream stimulation factor.
Immunogenetics
; 38(6): 430-6, 1993.
Article
in En
| MEDLINE
| ID: mdl-7691734
ABSTRACT
HLA-A and -B are expressed by most cell types, and their levels can be increased by treatment with interferons (IFNs). The relative basal levels of HLA-A and -B expression can vary, and HLA-B loci are induced much more strongly by IFNs. Constitutive activity is dependent on an upstream enhancer (ENH) which contains a rel (KBF, NF kappa B) binding motif, and induction is mediated by an interferon response element (IRE) which binds members of the IRF family. Reported here is the identification of a regulatory element, 'R', which overlaps the IRE of HLA-B loci, but which is absent from the equivalent region of HLA-A or H2 class I genes. The core of the element, CACGAG, is bound by a nuclear factor which is recognized by an antiserum raised against the upstream stimulation factor (USF), a member of the helix-loop-helix/leucine zipper family. The use of reporter gene constructs shows that mutation of the R element results in increased induction by IFN alpha in some cell lines, which appears to be due to competitive binding of USF with IRF proteins.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Genes, MHC Class I
/
HLA-B Antigens
/
Regulatory Sequences, Nucleic Acid
/
Gene Expression Regulation
/
DNA-Binding Proteins
Type of study:
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
Immunogenetics
Year:
1993
Type:
Article
Affiliation country:
United kingdom