PCR detection of ovine herpesvirus-2 DNA in Indonesian ruminants--normal sheep and clinical cases of malignant catarrhal fever.
Vet Microbiol
; 42(1): 45-52, 1994 Sep.
Article
in En
| MEDLINE
| ID: mdl-7839584
ABSTRACT
Malignant catarrhal fever (MCF), a fatal viral disease of cattle and other large ruminants, has a worldwide distribution. There are two forms of the disease, one of which, is caused by Alcelaphine herpesvirus-1 (AHV-1) and is derived from wildebeest. The other form is associated with domestic sheep and is caused by ovine herpesvirus-2 (OHV-2). The disease in Indonesia is sheep-associated with the preferred livestock of this area, Balinese cattle (Bos javanicus) and water buffalo (Bubalus bubalis), both highly susceptible to SA-MCF. The incidence in these species is thought to be high but the prevalence and economic losses attributable to SA-MCF have been difficult to assess. a polymerase chain reaction (PCR) test, based on a cloned OHV-2 gene sequence, was successfully applied to the detection of OHV-2 DNA in normal sheep and animals affected with SA-MCF. OHV-2 DNA was detected in eleven confirmed cases of SA-MCF and in the peripheral blood leucocyte (PBL) fraction of six latently infected sheep. These findings have confirmed that the PCR can be of value in establishing a diagnosis of MCF and that the aetiological agent of MCF in Indonesia is OHV-2. The amplification of DNA from the PBL of goats suggests that they are infected with a similar or identical herpesvirus.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Ruminants
/
DNA, Viral
/
Herpesviridae
/
Malignant Catarrh
Type of study:
Diagnostic_studies
/
Risk_factors_studies
Limits:
Animals
Country/Region as subject:
Asia
Language:
En
Journal:
Vet Microbiol
Year:
1994
Type:
Article
Affiliation country:
Indonesia