Retinoic acid modulation of alpha(1-->2) fucosyltransferase activity and sensitivity of tumor cells to LAK-mediated cytotoxicity.
In Vitro Cell Dev Biol
; 29A(2): 140-4, 1993 Feb.
Article
in En
| MEDLINE
| ID: mdl-8473271
ABSTRACT
We examined the effects of all-trans retinoic acid (RA) on alpha(1-->2) fucosyltransferase activity and sensitivity to LAK-mediated cytotoxicity in two rat colon carcinoma cell lines differing by their glycosylation state and their tumorigenic potential. RA induced a decrease in alpha(1-->2) fucosyltransferase activity in the more tumorigenic variant PROb. Fucosyltransferase mRNA levels were not affected by RA treatment in PROb cells, suggesting a posttranscriptional control. This inhibition was accompanied by a decreased expression of fucosylated membrane glycoconjugates and by a significant increase in the sensitivity to LAK-mediated cytotoxicity. REGb cells, which exhibited a very low enzymatic activity and very few fucosylated glycoconjugates, were more sensitive to LAK-lysis than PROb cells and were not affected by RA treatment.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Tretinoin
/
Killer Cells, Lymphokine-Activated
/
Cytotoxicity, Immunologic
/
Fucosyltransferases
Type of study:
Diagnostic_studies
Limits:
Animals
Language:
En
Journal:
In Vitro Cell Dev Biol
Year:
1993
Type:
Article
Affiliation country:
France