Demonstration of infectious salmon anaemia (ISA) viral antigens in cell cultures and tissue sections.
Vet Res
; 26(5-6): 499-504, 1995.
Article
in En
| MEDLINE
| ID: mdl-8581029
ABSTRACT
Rabbit polyclonal antibodies and mouse monoclonal antibodies (MAb) directed against infectious salmon anaemia virus (ISAV) were produced using a virus prepared in a newly established cell line culture developed from Atlantic salmon head kidney (SHK-1) cells. These antibodies were used to establish an indirect fluorescent antibody test for the detection of viral antigens in cell cultures and tissue cryosections. Specific fluorescence was detected in ISAV-infected cell cultures using rabbit polyclonal and MAb. One selected MAb produced specific staining for ISAV in the tissue sections from all the examined organs of ISAV-infected Atlantic salmon 20 d post infection. Fluorescence was mainly found in the endothelial cells and in single cells scattered throughout the parenchyma of the organs.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Salmon
/
Lentivirus Infections
/
Fish Diseases
/
Anemia
/
Antigens, Viral
Type of study:
Diagnostic_studies
Limits:
Animals
Language:
En
Journal:
Vet Res
Journal subject:
MEDICINA VETERINARIA
Year:
1995
Type:
Article
Affiliation country:
Norway