Human intervertebral disc cells from the annulus: three-dimensional culture in agarose or alginate and responsiveness to TGF-beta1.

Gruber, H E; Fisher, E C; Desai, B; Stasky, A A; Hoelscher, G; Hanley, E N

Gruber, H E; Fisher, E C; Desai, B; Stasky, A A; Hoelscher, G; Hanley, E N.

Affiliation

Gruber HE; Department of Orthopaedic Surgery, Carolinas Medical Center, Charlotte, North Carolina 28232, USA. hgruber@carolinas.org

Exp Cell Res ; 235(1): 13-21, 1997 Aug 25.

Article in En | MEDLINE | ID: mdl-9281347

Subject(s)

Collagen/biosynthesis; Intervertebral Disc/cytology; Proteoglycans/biosynthesis; Transcription, Genetic; Adult; Alginates; Biglycan; Biocompatible Materials; Cell Culture Techniques/methods; Cell Division/drug effects; Cells, Cultured; Chondroitin Sulfates/analysis; Chondroitin Sulfates/biosynthesis; Collagen/analysis; Decorin; Extracellular Matrix Proteins; Female; Gene Expression; Glucuronic Acid; Hexuronic Acids; Humans; Immunohistochemistry; Intervertebral Disc/metabolism; Intervertebral Disc/pathology; Keratan Sulfate/analysis; Keratan Sulfate/biosynthesis; Kinetics; Sepharose; Transforming Growth Factor beta/pharmacology

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Collection: 01-internacional Database: MEDLINE Main subject: Proteoglycans / Transcription, Genetic / Collagen / Intervertebral Disc Limits: Adult / Female / Humans Language: En Journal: Exp Cell Res Year: 1997 Type: Article Affiliation country: United States

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