Amyloid beta-protein (A beta) associated with lipid molecules: immunoreactivity distinct from that of soluble A beta.
FEBS Lett
; 420(1): 43-6, 1997 Dec 22.
Article
in En
| MEDLINE
| ID: mdl-9450547
ABSTRACT
We previously identified a novel amyloid beta-protein (A beta), that binds to GM1 ganglioside, in brains exhibiting the early pathological changes of AD. In this study, we raised monoclonal antibodies, using membrane fractions containing abundant GM1 ganglioside-bound A beta as antigens. Monoclonal antibody 4396, produced in this study, immunoprecipitates A beta42 in the membrane fractions of brains with diffuse plaques, but does not react with soluble A beta42 or GM1 ganglioside. Furthermore, this antibody recognizes the A beta bound to lipid vesicles containing GM1 ganglioside, and unexpectedly, phosphatidylinositol. In contrast, a control anti-A beta monoclonal antibody does not recognize the A beta bound to these lipid vesicles. These results indicate that A beta associated with lipids has an immunoreactivity distinct from that of soluble A.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Amyloid beta-Peptides
/
Lipid Metabolism
/
G(M1) Ganglioside
Type of study:
Risk_factors_studies
Limits:
Animals
/
Female
/
Humans
Language:
En
Journal:
FEBS Lett
Year:
1997
Type:
Article
Affiliation country:
Japan