Development of a PCR assay for detection of Yersinia ruckeri in tissues of inoculated and naturally infected trout.
Appl Environ Microbiol
; 65(1): 346-50, 1999 Jan.
Article
in En
| MEDLINE
| ID: mdl-9872807
ABSTRACT
A PCR-based method was developed for the specific detection of Yersinia ruckeri in tissues of inoculated trout and naturally infected trout. No amplification products were obtained with other yersiniae, bacterial fish pathogens, or phylogenetically related bacteria (n = 34). The sensitivity of PCR detection was 60 to 65 bacterial cells per PCR tube, which was decreased to 10 to 20 cells by hybridization with a nonradioactive probe. The PCR assay proved to be as reliable as and faster than the conventional culture method for the detection of Y. ruckeri in infected trout tissues.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Yersinia
/
Polymerase Chain Reaction
/
Oncorhynchus mykiss
Type of study:
Diagnostic_studies
/
Evaluation_studies
Limits:
Animals
Language:
En
Journal:
Appl Environ Microbiol
Year:
1999
Type:
Article
Affiliation country:
Spain