Identification and characterization of transcriptional regulation of the Mannheimia haemolytica ferric uptake regulator.
Vet Microbiol
; 124(3-4): 298-309, 2007 Oct 06.
Article
en En
| MEDLINE
| ID: mdl-17544233
ABSTRACT
The ferric uptake regulator (Fur) is an iron-dependent transcriptional regulator that regulates genes related to iron acquisition, oxidative stress response, and various other functions. Transcription of fur is typically self-regulating and sensitive to iron and oxidative stress. Following the identification of a fur gene in the genome of the bovine pathogen Mannheimia haemolytica, an attempt was made to characterize the transcriptional control of M. haemolytica fur. Northern blotting, RT-PCR, and primer extension were done to determine that M. haemolytica fur is transcribed using three distinct promoters, two of which are located within the upstream fldA gene. The third promoter is located upstream of a conserved hypothetical protein and drives transcription of a tricistronic message. Quantitative real time PCR experiments indicated that unlike current models of Fur regulation, M. haemolytica fur transcription is unchanged by iron depletion at logarithmic phase and repressed by iron depletion at stationary phase.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Proteínas Represoras
/
Proteínas Bacterianas
/
Transcripción Genética
/
Regulación Bacteriana de la Expresión Génica
/
Mannheimia haemolytica
/
Hierro
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Vet Microbiol
Año:
2007
Tipo del documento:
Article
País de afiliación:
Estados Unidos