New and efficient method using Saccharomyces cerevisiae mutants for identification of siderophores produced by microorganisms.
Curr Genet
; 52(3-4): 187-90, 2007 Sep.
Article
en En
| MEDLINE
| ID: mdl-17665200
ABSTRACT
The separation and identification of siderophores produced by microorganisms is a time-consuming and an expensive procedure. We have developed a new and efficient method to identify siderophores using well-established Saccharomyces cerevisiae deletion mutants. The Deltafet3,arn strains fail to sustain growth, even when specific siderophores are supplied, and mutants are siderophore-specific Deltafet3,arn2 for triacetylfusarinine C (TAFC), Deltafet3,arn1,sit1 for ferrichrome (FC), and Deltafet3,sit1 for ferrioxamine B (FOB). The culture broth of Fusarium graminearum was separated by HPLC, and each peak was subjected to a plate assay using S. cerevisiae mutants. We have found that each peak contained specific siderophores produced by F. graminearum, and these coincided with reference siderophores. This method is quite novel because nobody tried this method to identify the siderophores. Furthermore, this method will save time and cost in the identification of siderophores produced by microorganisms.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Saccharomyces cerevisiae
/
Cromatografía Líquida de Alta Presión
/
Sideróforos
/
Proteínas de Saccharomyces cerevisiae
/
Fusarium
/
Mutación
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Curr Genet
Año:
2007
Tipo del documento:
Article