Grapevine cell early activation of specific responses to DIMEB, a resveratrol elicitor.
BMC Genomics
; 10: 363, 2009 Aug 06.
Article
en En
| MEDLINE
| ID: mdl-19660119
ABSTRACT
BACKGROUND:
In response to pathogen attack, grapevine synthesizes phytoalexins belonging to the family of stilbenes. Grapevine cell cultures represent a good model system for studying the basic mechanisms of plant response to biotic and abiotic elicitors. Among these, modified beta-cyclodextrins seem to act as true elicitors inducing strong production of the stilbene resveratrol.RESULTS:
The transcriptome changes of Vitis riparia x Vitis berlandieri grapevine cells in response to the modified beta-cyclodextrin, DIMEB, were analyzed 2 and 6 h after treatment using a suppression subtractive hybridization experiment and a microarray analysis respectively. At both time points, we identified a specific set of induced genes belonging to the general phenylpropanoid metabolism, including stilbenes and hydroxycinnamates, and to defence proteins such as PR proteins and chitinases. At 6 h we also observed a down-regulation of the genes involved in cell division and cell-wall loosening.CONCLUSIONS:
We report the first large-scale study of the molecular effects of DIMEB, a resveratrol inducer, on grapevine cell cultures. This molecule seems to mimic a defence elicitor which enhances the physical barriers of the cell, stops cell division and induces phytoalexin synthesis.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Estilbenos
/
Vitis
/
Beta-Ciclodextrinas
Idioma:
En
Revista:
BMC Genomics
Asunto de la revista:
GENETICA
Año:
2009
Tipo del documento:
Article
País de afiliación:
Italia