The transcription factors BEL1 and SPL are required for cytokinin and auxin signaling during ovule development in Arabidopsis.
Plant Cell
; 24(7): 2886-97, 2012 Jul.
Article
en En
| MEDLINE
| ID: mdl-22786869
ABSTRACT
Hormones, such as auxin and cytokinin, are involved in the complex molecular network that regulates the coordinated development of plant organs. Genes controlling ovule patterning have been identified and studied in detail; however, the roles of auxin and cytokinin in ovule development are largely unknown. Here we show that key cytokinin pathway genes, such as isopentenyltransferase and cytokinin receptors, are expressed during ovule development. Also, in a cre1-12 ahk2-2 ahk3-3 triple mutant with severely reduced cytokinin perception, expression of the auxin efflux facilitator PIN-FORMED 1 (PIN1) was severely reduced. In sporocyteless/nozzle (spl/nzz) mutants, which show a similar phenotype to the cre1-12 ahk2-2 ahk3-3 triple mutant, PIN1 expression is also reduced. Treatment with the exogenous cytokinin N(6)-benzylaminopurine also altered both auxin distribution and patterning of the ovule; this process required the homeodomain transcription factor BELL1 (BEL1). Thus, this article shows that cytokinin regulates ovule development through the regulation of PIN1. Furthermore, the transcription factors BEL1 and SPL/NZZ, previously described as key regulators of ovule development, are needed for the auxin and cytokinin signaling pathways for the correct patterning of the ovule.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Proteínas de Transporte de Membrana
/
Reguladores del Crecimiento de las Plantas
/
Proteínas Represoras
/
Factores de Transcripción
/
Proteínas Nucleares
/
Transducción de Señal
/
Arabidopsis
/
Proteínas de Homeodominio
/
Proteínas de Arabidopsis
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Plant Cell
Asunto de la revista:
BOTANICA
Año:
2012
Tipo del documento:
Article
País de afiliación:
Italia